Isolation, characterization, and chromosomal localization of the porcine calcitonin receptor gene. Identification of two variants of the receptor generated by alternative splicing.
作者： B A Hemmings , R Fries , H Y Lin , P Cron , S Solinas-Toldo
DOI: 10.1016/S0021-9258(17)32201-9
关键词:
摘要: The gene encoding the calcitonin receptor (CTR) was isolated from a porcine kidney epithelial cell line (LLC-PK1) genomic library and found to span approximately 70 kilobases. Analysis of sequence revealed that CTR mRNA encompasses 14 exons with 12 protein. Two splicing acceptor sites separated by 48 nucleotides were in intron 7. expression two species LLC-PK1 cells subsequently confirmed reverse transcription-polymerase chain reaction (RT-PCR) DNA sequencing. In shorter (CTR-1a) is 1,000 times more abundant than longer variant (CTR-1b), as estimated competitive RT-PCR. transcription initiation site mapped primer extension, S1 nuclease, RT-PCR analysis. proximal promoter region 500 base pair GC-rich (66%) CpG-rich (CpG/GpC ratio 0.71). Transient transfection promoter-luciferase chimeras led luciferase activity. chromosome band 9q11-q12.
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