The phosphorylation of the N protein could affect PRRSV virulence in vivo.
作者： Yao Chen , Zhiqing Yu , Heyou Yi , Yingfang Wei , Xiaoliang Han
DOI: 10.1016/J.VETMIC.2019.03.018
关键词:
摘要: The porcine respiratory and reproductive syndrome virus (PRRSV) nucleocapsid (N) protein is a multiphosphorylated protein.It has been proved that the phosphorylation of N could regulate growth ability PRRSV in Marc-145 cells. However, further investigation needed to determine whether affect virulence piglets. In this study, we confirmed mutations impair replication primary macrophages (PAMs) as they did animal experiments suggested pathogenicity mutated (A105-120) was significantly reduced compared with parent strain (XH-GD). Our results contributes virulence. This study first identify specific modification involved pathogenicity. Mutation PTMs sites also novel way attenuate be marker vaccine. conclusion, our will improve understanding molecular mechanisms
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