Phenotype profile of blood lymphocytes in bronchiolitis obliterans after lung transplantation

摘要: Bronchiolitis obliterans (BO) remains the major complication in long-term survivors with lung transplants, occurring up to 30% of them. As a non-invasive follow-up recipients, we studied phenotype peripheral blood lymphocyte subsets. Using flow cytometric analysis, could define specific pattern during BO. The most important findings were 1) disappearance CD19+ B cell population, despite normal or increased immunoglobulin levels; 2) marked decrease CD4+/CD8+ ratio; 3) dramatic increase phenotypic cytotoxic effector T cells CD8+S6F1+ (MHC Class I-restricted allocytotoxicity) and CD3+CD4-CD8- I-non restricted allocytotoxicity); 4) CD4+CD29+ (helper/inducer cell) CD4+CD45RA+ (suppressor/inducer ratio associated loss suppressor/inducer cells. Moreover, have shown that maintenance triple immunosuppressive regimen consisted cyclosporin, prednisolone azathioprine, did not affect relative distribution subsets, except for subset was slightly decreased under therapy. Thus, using selected combination membrane antigens, sequential prospective testing should be useful lung-transplanted patients predict halt progressive course towards