Sequence analysis of low-molecular-weight RNAs by the use of non-radioactive labeling.

摘要: A new procedure for non-radioactive labeling of the 3'-terminal -OH was developed to facilitate sequencing works on low-molecular-weight RNAs. At first, biotinylated pCp (cytidine 3',5'-bisphosphate) synthesized and ligated yeast tRNA(Phe) with aid RNA ligase. Although efficiency high enough, this resulted in a lack sequence ladders around 10 nucleotides. longer oligonucleotide, p(dC)(dT)16-Biotin designed overcome defect proved be better 3'-labeling probe.