Spatially resolved, highly multiplexed RNA profiling in single cells

摘要: INTRODUCTION: The copy number and in- tracellular localization of RNA are important regulators gene expression. Measurement these properties at the transcriptome scale in single cells will give answers to many ques- tions related expression regulation. Single-molecule imaging approaches, such as single-molecule fluorescence situ hybrid- ization(smFISH), powerful toolsforcount- ing mappingRNA; however, species that can be simultaneously im- aged individual has been limited. This makes it challenging perform transcriptomic analysis a spatially resolved manner. Here, we report multiplexed error- robust FISH (MERFISH), aging method allows thousands imaged by using combinatorial labeling with encoding schemes capable detecting and/or correct- errors. RATIONALE: We labeled each cellular set probes, which contain targeting sequences bind readout fluorescently la- beled probes. Each is encodedwithaparticular combinationofread- out sequences. used successive rounds hybridization imaging, differ- ent probe, identify se- quences bound decode RNA. In principle, al- lows detectable