Photoswitchable Antigen−Antibody Interactions Studied by Impedance Spectroscopy

摘要: A thiolated dinitrospiropyran (1a) is assembled as a monolayer onto Au electrode. The exhibits reversible photoisomerizable properties, and irradiation of the 1a state, 360 nm 495 nm, regenerates interface acts antigen for dinitrophenyl antibody, DNP-Ab, while 1b state lacks an affinity DNP-Ab. This stimulates association antibody to interface, photoisomerization allows one wash off DNP-Ab regenerate assembly by secondary illumination process. binding or dissociation from are studied impedance spectroscopy, in presence absence solubilized redox probe. derived equivalent circuits ena...