GUIDANCE: a web server for assessing alignment confidence scores

摘要: Evaluating the accuracy of multiple sequence alignment (MSA) is critical for virtually every comparative analysis that uses an MSA as input. Here we present GUIDANCE web-server, a user-friendly, open access tool identification unreliable regions. The web-server accepts input set unaligned sequences. server aligns sequences and provides simple graphic visualization confidence score each column, residue alignment, using color-coding scheme. method generic user allowed to choose algorithm (ClustalW, MAFFT PRANK are supported) well any type molecular (nucleotide, protein or codon sequences). implements two different algorithms evaluating scores: (i) heads-or-tails (HoT) method, which measures uncertainty due co-optimal solutions; (ii) robustness guide-tree uncertainty. projects scores onto points columns unreliably aligned. These can be automatically removed in preparation downstream analyses. freely available use at http://guidance.tau.ac.il.