Lectin–carbohydrate interactions on nanoporous gold monoliths

摘要: Monoliths of nanoporous gold (np-Au) were modified with self-assembled monolayers octadecanethiol (C18-SH), 8-mercaptooctyl α-D-mannopyranoside (αMan-C8-SH), and 8-mercapto-3,6-dioxaoctanol (HO-PEG2-SH), the loading was assessed using thermogravimetric analysis (TGA). Modification mixed SAMs containing αMan-C8-SH (at a 0.20 mole fraction in SAM forming solution) either octanethiol or HO-PEG2-SH also investigated. The np-Au monoliths bind lectin Concanavalin A (Con A), additional mass due to bound protein TGA analysis. comparison traces measured before after exposure Con showed that non-specific binding minimal. In contrast, significant loss non-specifically adsorbed A. attributed bare monoliths. revealed pure αMan-C8-SH. use losses determined by compare surfaces is specific for but shows contribution from adsorption octanethiol. Minimal immunoglobulin G (IgG) peanut agglutinin (PNA) towards mannoside demonstrated. greater found onto monolith than IgG PNA, signifying mannose presenting retain selectivity data provide evidence can be eluted flowing solution methyl through structure. presence proteins on surface confirmed atomic force microscopy (AFM). results highlight potential application carbohydrate glycoscience glycotechnology demonstrate they used capture release quantities.