Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability.
作者： Xiao Wang , Zhanping Zhou
DOI: 10.1186/S12896-021-00693-8
关键词:
摘要: … In this study, we constructed a series of proposed thermostable mutants by rational design. Finally, we obtained PelN-G241A and PelN-G241V with improved thermostability but without …
biomedcentral.com
sci-hub.st 参考文章(40)
Susanne Melzer, Christian Sonnendecker, Christina Föllner, Wolfgang Zimmermann, Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp. FEBS Open Bio. ,vol. 5, pp. 528- 534 ,(2015) , 10.1016/J.FOB.2015.06.002
Sylvie Reverchon, William Nasser, Dickeya ecology, environment sensing and regulation of virulence programme. Environmental Microbiology Reports. ,vol. 5, pp. 622- 636 ,(2013) , 10.1111/1758-2229.12073
Xin Yin, Jian-Fang Li, Chun-Juan Wang, Die Hu, Qin Wu, Ying Gu, Min-Chen Wu, Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis. Applied Microbiology and Biotechnology. ,vol. 99, pp. 10047- 10056 ,(2015) , 10.1007/S00253-015-6889-2
Sameh Ben Mabrouk, Nushin Aghajari, Mamdouh Ben Ali, Ezzedine Ben Messaoud, Michel Juy, Richard Haser, Samir Bejar, Enhancement of the thermostability of the maltogenic amylase MAUS149 by Gly312Ala and Lys436Arg substitutions Bioresource Technology. ,vol. 102, pp. 1740- 1746 ,(2011) , 10.1016/J.BIORTECH.2010.08.082
Chaoning Liang, Xiwu Gui, Cheng Zhou, Yanfen Xue, Yanhe Ma, Shuang-Yan Tang, Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming. Applied Microbiology and Biotechnology. ,vol. 99, pp. 2673- 2682 ,(2015) , 10.1007/S00253-014-6091-Y
Karin L Heckman, Larry R Pease, Gene splicing and mutagenesis by PCR-driven overlap extension Nature Protocols. ,vol. 2, pp. 924- 932 ,(2007) , 10.1038/NPROT.2007.132
Giorgio Colombo, Kenneth M. Merz, Stability and Activity of Mesophilic Subtilisin E and Its Thermophilic Homolog: Insights from Molecular Dynamics Simulations Journal of the American Chemical Society. ,vol. 121, pp. 6895- 6903 ,(1999) , 10.1021/JA990420S
Mouyong Zou, Xuezhi Li, Wenjing Shi, Fenfen Guo, Jian Zhao, Yinbo Qu, Improved production of alkaline polygalacturonate lyase by homologous overexpression pelA in Bacillus subtilis Process Biochemistry. ,vol. 48, pp. 1143- 1150 ,(2013) , 10.1016/J.PROCBIO.2013.05.023
Huilin Wang, Xiaoman Li, Yanhe Ma, Jiangning Song, None, Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli Process Biochemistry. ,vol. 49, pp. 69- 76 ,(2014) , 10.1016/J.PROCBIO.2013.10.001
Zhanping Zhou, Shuangzhi Zhao, Shouquan Wang, Xiaoman Li, Li Su, Yanhe Ma, Jian Li, Jiangning Song, Extracellular Overexpression of Chitosanase from Bacillus sp. TS in Escherichia coli Applied Biochemistry and Biotechnology. ,vol. 175, pp. 3271- 3286 ,(2015) , 10.1007/S12010-015-1494-5