Parathyroid hormone-responsive clonal cell lines from rat osteosarcoma

摘要: Several clonal cell lines from a transplantable rat osteosarcoma, selected on the basis of parathyroid hormone (PTH)-sensitive adenylate cyclase, were established in culture. Bovine PTH-(1-84) (0.1 microM) stimulation cyclase varied among clones 8-fold to none. The level PTH response was stable property each line that retained through numerous passages vitro (nearly 3 yr oldest clone). Highly PTH-responsive had cuboidal-eliptoid morphology and differed nonresponsive lines, which more fibroblastic appearance. responsiveness correlated with several properties, presumably associated osteoblastic phenotype: elevated alkaline phosphatase activity, synthesis gamma-carboxyglutamic acid-containing bone protein, production mineralized tumors host rats. (1.0 nM; 24 h) reduced activity by 40% when tested responsive clone. acid various uniformly low. These osteosarcoma-derived are thus seem reflect phenotypic heterogeneity observed tumor situ. They could be useful studies expression, action, possible relationship between two.