Growth of Trypanosoma cruzi in cultures of chick embryo cells, and effects of furazolidone and tris(p-aminophenyl)carbonium chloride.

摘要: SYNOPSIS. Monolayers of cells coverslips were produced by culturing known numbers trypsinized chick in growth medium (solution 199 plus 20% calf serum) at 37 C for 2 days. The fluid was then replaced with maintenance 5% containing various T. cruzi and the preparations incubated 33 5 days; fresh substituted on 2nd or 3rd day. inocula parasites obtained from cruzi-cell cultures, supplemented 2% sterile NNN overlay, cultures. The extracellular parasites, proportions infected cells, percentage distribution relative to number intracellular leishmanial bodies determined days 3 parasite cultivation many experiments. Analyses data gave following results. Extracellular increased 2- 14-fold during first days, depending upon source size inocula, 10- 20-fold last Cell infection continued throughout incubation daily rates 1.4-4.5%; 8-22% became incubation. Intracellular reflected most clearly increases proportion having >10 bodies. This increase about incubation. A useful test procedure assessing antiparasitic action embryo cell toxicity drugs is illustrated furazolidone tris (p-aminophenyl)carbonium chloride.