Evaluation of epithelial and keratin markers in glioblastoma multiforme: an immunohistochemical study.

摘要: Objective Poorly differentiated metastatic carcinoma may be difficult to distinguish histologically from high-grade astrocytic malignant neoplasms, particularly on small open or stereotactic biopsy specimens. Previous authors have reported that a subset of glioblastoma multiforme (GBM) variably stains with cytokeratin immunomarkers. The examined panel epithelial and keratin antibodies by paraffin immunohistochemistry evaluate the immunophenotype GBM for these markers determine what combination immunostains would optimal in distinguishing carcinoma. Methods Twenty-three patients (age range, 19-86 years; mean, 63.4 14 men 9 women) 22 26-77 58.1 7 15 brain were studied immunostains, including glial fibrillary acid protein (GFAP), Ber-EP4, antikeratin monoclonal AE1/3, CAM 5.2 cytokeratins (CK7) 20 (CK20). Sites origin tumors included lung (n = 11), breast 5), endometrium 1), prostate colon presumed kidney unknown 2). Results All GBMs stained positive GFAP (100%), all but 1 (95.7%) AE1/3. Only rare focal immunoreactivity was observed single case (4.3%), CK7 CK20 (4.3%). Immunoreactivity Ber-EP4 not any (0.0%). cases AE1/3 (100%) (100%). Variable staining carcinomas (17 22, 77.3%), (11 50.0%), (9 40.9%). Three showed GFAP-positive cells (13.6%). Conclusions Based aforementioned results, CAM5.2, most useful differentiating poorly GBM. A significant number stain some markers, particular Because poor specificity GBM, it should used differentiate 2 entities.