Collagen biosynthesis. Characterization of subcellular fractions from embryonic chick fibroblasts and the intracellular localization of protocollagen prolyl and protocollagen lysyl hydroxylases

摘要: 1. Subcellular fractions of freshly isolated matrix-free embryonic chick tendon and sternal cartilage cells have been characterized by chemical analysis, electron microscopy the location specific marker enzymes. These data indicate to be a high degree purity comparable with those obtained from other tissues, e.g. liver kidney. 2. When homogenates were assayed for protocollagen prolyl hydroxylase lysyl activities, addition Triton X-100 (0.1%, w/v) was found stimulate enzyme activities up 60% suggesting that enzymes probably membrane-bound. 3. Assay subcellular differential centrifugation activity indicated highest in microsomal fraction. Similar results activity. 4. Submicrosomal discontinuous sucrose-gradient two associated almost exclusively rough endoplasmic reticulum fraction both cells.