Growth Limiting pH, Water Activity, and Temperature for Neurotoxigenic Strains of Clostridium butyricum

摘要: Some rare strains of Clostridium butyricum carry the gene encoding botulinal type E neurotoxin and must be considered as possible hazards in certain types food. The limiting growth conditions for C. were determined peptone yeast glucose starch (PYGS) broth incubated anaerobically at 30°C up to 42 days. minimum pH values permitting depended on acidulant strain. Organic acids more effective inhibiting than HCl expected. lowest which toxigenic nontoxigenic was observed acidified with 4.1 4.2, respectively. In organic acids, however, varied between 4.4 5.1 depending acid concentration. water activity 0.96. temperatures (ca 10-11°C) somewhat higher non-toxigenic ones (8°C). It concluded that control food industry needs allow greater tolerance this species compared proteolytic botulinum.