Whole-animal mounts of Caenorhabditis elegans for 3D imaging using atomic force microscopy.

摘要: Abstract The 3D surface of Caenorhabditis elegans was imaged at nanometer resolution using atomic force microscopy (AFM). Oscillation a medium stiffness silicon AFM cantilever the upper second amplitude peak, typically 6 times above fundamental frequency, vastly improved image quality on moist, sticky, and soft worms. Whole-animal mounts normal double-headed mutants nematode worm were prepared scanned. Well-preserved anatomical features including annuli, furrows, alae, rows never before seen nanometer-sized pores dotting molted worm's outermost coat resolved. or struts This method represents simple rapid new approach for nanometer-resolved imaging analysis whole-animal specimens C. . From Clinical Editor In this interesting article authors describe sampling to allow better images such as elegans. would generate more information in future may be useful differentiating even individual animals with different genetic backgrounds.