Identification of novel innate immune genes by transcriptional profiling of macrophages stimulated with TLR ligands.

作者: Ivana V. Yang , Weiwen Jiang , Holly R. Rutledge , Brad Lackford , Laura A. Warg

DOI: 10.1016/J.MOLIMM.2011.05.015

关键词:

摘要: Toll-like receptors (TLRs) are key in innate immunity and trigger responses following interaction with pathogen-associated molecular patterns (PAMPs). TLR3, TLR4 TLR9 recognize double stranded RNA, lipopolysaccharide (LPS) CpG DNA, respectively. These differ importantly downstream adaptor molecules. signals through MyD88 TRIF; contrast, the TLR3 pathway involves only TRIF while solely MyD88. To determine how differences signaling could influence gene expression immunity, were determined for RAW264.7 macrophage cell line stimulated LPS, poly (I:C), or DNA. Gene profiles 6 24h post-stimulation analyzed to genes, pathways transcriptional networks induced. As these experiments showed, number extent of genes expressed varied stimulus. LPS (I:C) induced an abundant array cells at 6h treatment DNA many fewer. By analyzing data pathways, we prioritized differentially respect those common three TLR ligands as well shared by but not The importance changes was demonstrated indicating that RNA interference-mediated inhibition two identified this analysis, PLEC1 TPST1, reduced IL-6 production J774A.1 macrophages LPS. Together, findings delineate response different PAMPs identify new have previously been implicated immunity.

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