Biochemical Characterization of the Fungal Plasma Membrane ATPase, An Electrogenic Proton Pump

摘要: From a variety of flux and electrophysiological studies, it has become clear that there is rapid physiologically important circulation protons across the fungal plasma membrane (Eddy, 1978; Goffeau Slayman, 1981). Protons are pumped outwards by primary ATPase, generating both pH gradient (up to 3 units) potential (150 250 mV, inside negative), return inwards way H+-dependent cotransport systems for sugars, amino acids, inorganic ions (Fig. 1). A major goal research during past few years been establish molecular structure ATPase. In particular, reason ask whether belongs F0F1 class mitochondrial, chloroplast, bacterial ATPases (which capable ATP-driven proton translocation, even though they usually function in opposite direction synthesize ATP), or more closely related Na+/K+-ATPase, Ca2+-ATPase H+/K+-ATPase animal cells act “transport” pump cations their respective membranes).