Genetic variation in radish (Raphanus sativus L.) germplasm from Pakistan using morphological traits and RAPDs

摘要: Genetic diversity of 30 radish (Raphanus sativus L.) accessions was investigated at the phenotypic level with morphological characters and DNA using random amplified polymorphic (RAPD) technique. Thirty-six morpho-physiological traits were recorded from seedling stage to harvest. The 31 primers used generated 202 RAPD bands, which 158 (78.2%) polymorphic. Multivariate procedures classify germplasm on basis fragments. Dendrograms for Euclidean distance data Nei's genetic markers. Phenotypically, all classified into four major groups corresponding different forms cultivated radish. existing within each these suggested that they should be discriminated three botanical convarieties, sativusT (large-rooted), caudatus (pod-type) oleifer (oilseed-type). Clustering did not show any pattern association between collection sites. Instead, landrace associated their similarities horticultural uses. On other hand, intra-specific relationships several based analysis related primarily sites rather than affinities. polymorphism exhibited by various convarieties could exploited in mapping populations tag economically important traits. These genotypes also serve as a useful source root, leaf, pod seed. This preliminary study traditional landraces Pakistan provides information regarding potential.