Monoclonal antibodies specific for Hantaan virus.

摘要: Abstract Six hybridoma cell line producing monoclonal antibodies to Hantaan virus were established by fusion of NS-1 mouse myeloma cells with spleen mice immunized strain 76-118. The specificity these was immunoblotting analysis and immunofluorescence. Five the clones reacted antigens on surface in cytoplasm, one clone a determinant expressed only cytoplasm infected cells. Two produced that Mr 50,000 polypeptide virus-infected cellular extracts purified preparations. used examine antigenic relationship among strains between Prospect Hill nephropathia epidemica. Three capable distinguishing Lee 760-118 three additional determinants shared both strains. None six or