Acute memory phase of sevoflurane preconditioning is associated with sustained translocation of protein kinase C-α and ε, but not δ, in isolated guinea pig hearts

摘要: BACKGROUND AND OBJECTIVE Anaesthetic preconditioning (APC) exerts cardioprotective effects by reducing infarct size and improving recovery of contractile function after ischaemia-reperfusion. The interval between brief exposure to volatile anaesthetic sustained ischaemia, the acute memory phase, is dependent on intracellular signalling mediating this cardioprotection. Intramyocyte translocation protein kinase C (PKC) known be a key mediator in APC. We examined relationship time frame phase sevoflurane intramyocyte PKC-alpha, delta epsilon particulate fraction. METHODS Isolated perfused guinea pig hearts were subjected 30 min ischaemia 120 reperfusion. APC was elicited with one minimum alveolar concentration for 10 min. Washout times 10, 30, 60 90 studied. Contractile assessed monitoring left ventricular developed pressures. Infarct determined triphenyltetrazolium chloride staining. Translocation PKC western blot analysis. RESULTS After ischaemia-reperfusion, pressure recovered greater degree compared control washout min, but not Similarly, reduced Sustained PKC-alpha epsilon, delta, associated phase. CONCLUSION limited less than correlates preconditioning.