Human Serum Albumin Improves Arterial Dysfunction during Early Resuscitation in Mouse Endotoxic Model via Reduced Oxidative and Nitrosative Stresses

摘要: Human serum albumin (HSA) is used as a resuscitation fluid in sepsis. This study investigated the potential protective properties of HSA on vascular function mouse endotoxic model terms oxidative and nitrosative stresses. Swiss mice were treated with either lipopolysaccharide (LPS) (50 mg/kg i.p.) or vehicle. One five hours later, infused (4%, 10 ml/kg), normal saline (0.9% NaCl, 30 no fluid. Six after treatment, reactivity was assessed aortae small mesenteric arteries. Measurements NO superoxide anion (O2−) by spin trapping nuclear factor (NF)-κB, inducible synthase (iNOS), peroxynitrite Western blotting immunohistochemical studies conducted. partially prevented reduction blood pressure induced LPS completely both hyporeactivity to phenylephrine myogenic tone well endothelial dysfunction endotoxin. associated decreased up-regulation NF-κ B, iNOS, wall. LPS-induced tissue increases O2− production HSA. These data demonstrate effect treatment experimental shock reducing inflammatory process leading stresses hyporeactivity.