Cloning, purification, and characterization of chitinase from Bacillus sp. DAU101.
作者： Y LEE , I PARK , J YOO , S CHUNG , Y LEE
DOI: 10.1016/J.BIORTECH.2006.09.048
关键词:
摘要: Abstract A chitinase encoding gene from Bacillus sp. DAU101 was cloned in Escherichia coli . The nucleotide sequencing revealed a single open reading frame containing 1781 bp and 597 amino acids with 66 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis zymogram. composed of three domains: catalytic domain, fibronectin III chitin binding domain. purified GST-fusion purification system. pH temperature optima the enzyme were 7.5 60 °C, respectively. metal ions, Zn 2+ , Cu Hg strongly inhibited activity. However, activity increased 1.4-fold Co Chisb could hydrolyze GlcNAc 2 to N -acetylglucosamine produced when derivatives used as substrate. This indicated that bifunctional enzyme, -acetylglucosaminase chitobiosidase. not glycol chitin, chitosan, or CMC, but hydrolyzed colloidal soluble chitosan.
elsevier.com
sci-hub.se 参考文章(35)
T Ohno, S Armand, T Hata, N Nikaidou, B Henrissat, M Mitsutomi, T Watanabe, A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037. Journal of Bacteriology. ,vol. 178, pp. 5065- 5070 ,(1996) , 10.1128/JB.178.17.5065-5070.1996
Jannick Dyrløv Bendtsen, Henrik Nielsen, Gunnar von Heijne, Søren Brunak, Improved Prediction of Signal Peptides: SignalP 3.0 Journal of Molecular Biology. ,vol. 340, pp. 783- 795 ,(2004) , 10.1016/J.JMB.2004.05.028
H. Nielsen, J. Engelbrecht, S. Brunak, G. von Heijne, Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering. ,vol. 10, pp. 1- 6 ,(1997) , 10.1093/PROTEIN/10.1.1
P. Tomme, Cellulose-binding domains : classification and properties The Enzyme Degradation of Insoluble Polysaccharides. pp. 142- 161 ,(1995)
A. V. Il'ina, O. Yu. Zueva, S. A. Lopatin, V. P. Varlamov, Enzymatic Hydrolysis of α-Chitin Applied Biochemistry and Microbiology. ,vol. 40, pp. 35- 38 ,(2004) , 10.1023/B:ABIM.0000010348.46961.E2
P. Tomme, R.A.J. Warren, N.R. Gilkes, Cellulose hydrolysis by bacteria and fungi Advances in Microbial Physiology. ,vol. 37, pp. 1- 81 ,(1995) , 10.1016/S0065-2911(08)60143-5
A. Pelletier, J. Sygusch, Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1. Applied and Environmental Microbiology. ,vol. 56, pp. 844- 848 ,(1990) , 10.1128/AEM.56.4.844-848.1990
T Watanabe, Y Ito, T Yamada, M Hashimoto, S Sekine, H Tanaka, The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation. Journal of Bacteriology. ,vol. 176, pp. 4465- 4472 ,(1994) , 10.1128/JB.176.15.4465-4472.1994
Colin Ratledge, Biochemistry of microbial degradation ,(2012)
B Henrissat, A Bairoch, New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochemical Journal. ,vol. 293, pp. 781- 788 ,(1993) , 10.1042/BJ2930781