A Highly Contiguous Genome for the Golden-Fronted Woodpecker ( Melanerpes aurifrons ) via Hybrid Oxford Nanopore and Short Read Assembly.

作者: Graham Wiley , Matthew J. Miller

DOI: 10.1534/G3.120.401059

关键词:

摘要: Woodpeckers are found in nearly every part of the world and have been important for studies biogeography, phylogeography, macroecology. Woodpecker hybrid zones often studied to understand dynamics introgression between bird species. Notably, woodpeckers gaining attention their enriched levels transposable elements (TEs) relative most other birds. This enrichment TEs may substantial effects on molecular evolution. However, comparative woodpecker genomes hindered by fact that no high-contiguity genome exists any Using assembly methods combining long-read Oxford Nanopore short-read Illumina sequencing data, we generated a highly contiguous Golden-fronted (Melanerpes aurifrons). The final is 1.31 Gb comprises 441 contigs plus full mitochondrial genome. Half represented 28 (contig L50), each these at least 16 Mb size N50). High recovery (92.6%) bird-specific BUSCO genes suggests our both relatively complete accurate. Over quarter (25.8%) consists repetitive elements, with 287 (21.9%) those assignable CR1 superfamily highest proportion repeats reported date. Our should improve evolution genomics allies. Additionally, bioinformatic resources used generate this were low-cost provide direction development high-quality animal biodiversity.

参考文章(57)
Xuesong Hu, Binghang Liu, Wei Fan, Nan Li, Hao Zhang, Zhenyu Li, Desheng Mu, Yujian Shi, Jianying Yuan, Yanxiang Chen, Estimation of genomic characteristics by analyzing k-mer frequency in de novo genome projects arXiv: Genomics. ,(2013)
Bruce J. Walker, Thomas Abeel, Terrance Shea, Margaret Priest, Amr Abouelliel, Sharadha Sakthikumar, Christina A. Cuomo, Qiandong Zeng, Jennifer Wortman, Sarah K. Young, Ashlee M. Earl, Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement PLoS ONE. ,vol. 9, pp. e112963- ,(2014) , 10.1371/JOURNAL.PONE.0112963
Erick A. García-Trejo, Alejandro Espinosa de los Monteros, Ma. del Coro Arizmendi, Adolfo G. Navarro-Sigüenza, Molecular Systematics of The Red-Bellied and Golden-Fronted Woodpeckers The Condor. ,vol. 111, pp. 442- 452 ,(2009) , 10.1525/COND.2009.080017
Michael S. Husak, Amanda L. Husak, LATITUDINAL PATTERNS IN RANGE SIZES OF NEW WORLD WOODPECKERS The Southwestern Naturalist. ,vol. 48, pp. 61- 69 ,(2003) , 10.1894/0038-4909(2003)048<0061:LPIRSO>2.0.CO;2
Jérôme Fuchs, Jan I. Ohlson, Per G. P. Ericson, Eric Pasquet, Synchronous intercontinental splits between assemblages of woodpeckers suggested by molecular data Zoologica Scripta. ,vol. 36, pp. 11- 25 ,(2007) , 10.1111/J.1463-6409.2006.00267.X
TIM M. BLACKBURN, JOHN H. LAWTON, KEVIN J. GASTON, Patterns in the geographic ranges of the world's woodpeckers Ibis. ,vol. 140, pp. 626- 638 ,(2008) , 10.1111/J.1474-919X.1998.TB04708.X
Deanna Ojala, Julio Montoya, Giuseppe Attardi, tRNA punctuation model of RNA processing in human mitochondria Nature. ,vol. 290, pp. 470- 474 ,(1981) , 10.1038/290470A0
D. P. Mindell, M. D. Sorenson, D. E. Dimcheff, Multiple independent origins of mitochondrial gene order in birds Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 10693- 10697 ,(1998) , 10.1073/PNAS.95.18.10693
Guillaume Marçais, Carl Kingsford, A fast, lock-free approach for efficient parallel counting of occurrences of k-mers Bioinformatics. ,vol. 27, pp. 764- 770 ,(2011) , 10.1093/BIOINFORMATICS/BTR011
Stefan Kurtz, Adam Phillippy, Arthur L Delcher, Michael Smoot, Martin Shumway, Corina Antonescu, Steven L Salzberg, Versatile and open software for comparing large genomes Genome Biology. ,vol. 5, pp. 1- 9 ,(2004) , 10.1186/GB-2004-5-2-R12