Molecular Characterization of the Poly-ß-Hydroxybutyrate Biosynthetic Pathway of Alcaligenes Eutrophus H16

摘要: The poly-s-hydroxybutyrate biosynthetic pathway of Alcaligenes eutrophus H16 has been cloned into Escherichia coli, where it the subject a series molecular analyses. DNA sequence determined and analyzed for open reading frames secondary structure. In vivo transcription/translation studies have identified size gene products these aligned with specific frames. A putative promoter located approximately 300 bases upstream from start translation on basis deletion mutants consensus comparisons. PHB is made to levels approaching 90% cell weight, in large part due dosage effect multicopy plasmid which resides. production varies considerably response media alterations.