A system of indoleamine-accumulating neurons in the rabbit retina

摘要: The indoleamine-accumulating neurons of the rabbit retina were labeled by intraocular injection 5,7-dihydroxytryptamine (5,7-DHT). retinas fixed with 2.5% paraformaldehyde and 0.2% glutaraldehyde inspected fluorescence microscopy. Five kinds cell accumulated indoleamine. They to essentially same brightness remained so despite variations in concentration at which 5,7-DHT had been applied or duration its application. Experiments was incubated vitro gave identical results. To see whole shape cells, we visually guided micropipettes fluorescent bodies injected cells Lucifer yellow CH. study as a population, used new method is photochemically converted an insoluble diaminobenzidine product. dendrites all then simultaneously visible. Used together, these techniques revealed interrelated system neurons. All contribute processes dendritic plexus that lies inner margin plexiform layer. roughly 4 micron thick. It pierced stalks Muller occasionally interrupted ganglion bodies, where they extend above average Otherwise it fills much space junction layers. type 1 2 are amacrine nuclear have 5-8 radially branching primary horizontally across layer before descending join plexus. differ from each other body shape, morphology, course their within Each has "displaced" counterpart, morphology similar but located displaced separate functional elements because, contrast no (and hence can synaptic connections) outer part fifth kind (type 3) appears not described before. Its layer.(ABSTRACT TRUNCATED AT 400 WORDS)