Mutual interactions of E. andrei earthworm and pathogens during the process of vermicomposting.

摘要: Vermicomposting is a process by which earthworms together with microorganisms degrade organic wastes into humus-like material called vermicompost. This does not include thermophilic stage, and therefore, the possible presence of pathogens represents potential health hazard. To elucidate effect in selective reduction pathogens, grape marc substrate was artificially inoculated Escherichia coli, Enterococcus spp., thermotolerant coliform bacteria (TCB), Salmonella their during vermicomposting monitored. Various defense mechanisms eliminating earthworm gut were assumed to be involved pathogen reduction. Therefore, we followed expression three pattern recognition receptors (coelomic cytolytic factor (CCF), lipopolysaccharide-binding protein (LBP), Toll-like receptor (v-TLR)), two antimicrobial molecules (fetidin/lysenins lysozyme), heat shock HSP70. We detected significant decrease some LBP) all pathogen-inoculated substrates, increase CCF LBP spp.-inoculated substrate. At same time, assessed. observed accelerated E. TCB substrates compared that without earthworms. Moreover, differences between microbiome intestines determined high throughput sequencing. analysis revealed bacterial composition differed from content intestines, suggesting elimination specific species food passage through gut.