Immune and non-immune responses to monovalent low molecular weight penicilloyl—polylysines and penicilloyl—bacitracin in rabbits and guinea-pigs

摘要: Rabbits and guinea-pigs have been immunized with low molecular weight penicilloyl—polylysines (BPO1—PLL1 2, BPO6—PLL1 2) varying degrees of substitution monovalent penicilloyl—bacitracin (BPO1—BAC). The immune response to the penicilloyl (BPO) determinant has followed by skin tests, passive haemagglutination, cutaneous anaphylaxis (PCA), immunodiffusion immunoelectrophoresis. Maximally substituted 2 was found be non-immunogenic. Apparently BPO1—PLL1 which inhibits precipitation anti-BPO antibodies in vitro, induce delayed-type hypersensitivity for BPO most our random bred all strain guinea-pigs. delayed some sensitized hapten (BPO)-specific. very poorly immunogenic rabbits. Monovalent BPO1—BAC induced BPO-specific but not animals. good levels haemagglutinating rabbits. Truly compounds, such as or BPO—e-aminocaproate, do elicit antibody-dependent reactions anaphylactic Arthus reactions, inhibit them. On other hand, apparently PCA rabbits When administered higher doses, similar were produced non-immunized possibility is discussed that observed basic polypeptide antigens are due formation mixed `specific antibody—polypeptide—non-specific protein' complexes formed vivo electrostatic interaction.