Testosterone Supplementation Rescues Spermatogenesis and In Vitro Fertilizing Ability of Sperm in Kiss1 Knockout Mice.

摘要: Restoration of spermatogenesis and fertility is a major issue to be solved in male mammals with hypogonadotropic hypogonadism. Kiss1 knockout (KO) mice are postulated suitable animal model investigate if hormonal replacement rescues this severe reproductive hormone deficiency, because KO replicate the hypothalamic disorder causing The present study investigated whether testosterone supplementation was able restore vitro fertilization ability mice. To end, spermatogenesis, sperm, preimplantation development wild-type embryos inseminated were examined. newly generated showed infertility cryptorchidism. Subcutaneous for 6 weeks restored plasma intratesticular levels, elicited testicular descent, induced complete from spermatocytes elongated spermatids testis, resulting an increase epididymal sperm number testosterone-supplemented Epididymal derived normal ability, fertilized eggs development, while males failed impregnate females. These results suggest that failure mainly due lack production, capable fertilizing animals receive appropriate without local kisspeptin signaling testis epididymis. Thus, would showing hypogonadism genetic inactivation KISS1/Kiss1 gene.