SSR-based identification of genetic groups within European populations of Tuber aestivum Vittad

摘要: Tuber species are ectomycorrhizal ascomycetes establishing relationships with different host trees and forming hypogeous fruiting bodies known as truffles. Among species, aestivum Vittad. has a wide distributional range being found naturally all over Europe. Here, we performed large-scale population genetic analyses in T. to (i) investigate its diversity at the European scale, (ii) characterize structure test for presence of ecotypes (iii) shed light into demographic history. To reach these goals, 230 ascocarps from populations were genotyped using 15 polymorphic simple sequence repeat markers. We identified 181 multilocus genotypes four groups which did not show clear geographical separation; although, one them was present exclusively Southeast France, Italy Spain. Fixation index values between pairs generally high ranged 0.29 0.45. A significant deficit heterozygosity indicated expansion instead recent bottleneck, suggesting that is endangered Europe, even Mediterranean regions. Our study based on analysis suggests genetically distinct likely within present. In turn, this paves way future investigations aimed addressing biological and/or ecological factors have concurred shaping species. Present results should also implications truffle market since defining markers now possible least some specific groups.