Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
作者： Keith Pardee , Alexander A. Green , Melissa K. Takahashi , Dana Braff , Guillaume Lambert
DOI: 10.1016/J.CELL.2016.04.059
关键词:
摘要: The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled …
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