Isolation of foot-and-mouth disease virus from Japanese black cattle in Miyazaki Prefecture, Japan, 2000.

摘要: Four outbreaks of foot-and-mouth disease (FMD) occurred from March to May 2000 in Miyazaki and Hokkaido Prefectures, Japan. FMD virus isolation was achieved by sampling probang materials Japanese Black cattle the third case found Prefecture. The were inoculated bovine kidney (BK) thyroid cell cultures. CPE observed BK at two days post-inoculation. Specific amplified DNA segments for (FMDV) detected reverse transcriptase polymerase chain reaction culture fluid. FMDV identified as type O enzyme-linked immunosorbent assay (ELISA) antigen detection nucleotide sequence encoding VP1 determined. This is a strain that widespread Pan-Asia designated O/JPN/2000 World Reference Laboratory Pirbright Institute, England. report marks first