[37] The keilin-hartree heart muscle preparation

摘要: Publisher Summary This chapter describes the Keilin–Hartree's heart muscle preparation. The preparation has been used in study of intracellular respiration and as a starting material for large-scale isolations respiratory components. It is particulate suspension physically disintegrated mitochondrial membrane. manipulations are simple yield high. A number criteria may be determination activity. Among them, oxidation succinate or NADH by molecular oxygen useful informational. following describe manometric method oxidase polarographic procedure oxidase. Keilin originally employed his studies respiration. Later Hartree standardized Subsequently, because availability high-speed centrifuges adaptations special purposes, some variances have introduced. Four methods described this chapter.