Accelerated solvent extraction of carotenoids from: Tunisian Kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.)

摘要: Extraction of carotenoids from biological matrices and quantifications remains a difficult task. Accelerated solvent extraction was used as an efficient process for three fruits cultivated in Tunisia: kaki (Diospyros L.), peach (Prunus persica L.) apricot armeniaca L.). Based on design experiment (DoE) approach, using binary consisting methanol tetrahydrofuran, we could identify the best conditions being 40°C, 20:80 (v:v) methanol/tetrahydrofuran 5 min time. Surprisingly likely due to high pressure (103 bars), these appeared be ones both extracting xanthophylls such lutein, zeaxanthin or β-cryptoxanthin carotenes β-carotene, which present quite different polarities. Twelve surface responses were generated zeaxanthin, β-carotene kaki, apricot. Further LC-MS analysis allowed comparisons profiles between fruits.