Yeast Pab1 Interacts with Rna15 and Participates in the Control of the Poly(A) Tail Length In Vitro

摘要: In Saccharomyces cerevisiae, the single poly(A) binding protein, Pab1, is major ribonucleoprotein associated with tails of mRNAs in both nucleus and cytoplasm. We found that Pab1 interacts Rna15 two-hybrid assays coimmunoprecipitation experiments. Overexpression PAB1 partially but specifically suppressed rna15-2 mutation vivo. RNA15 codes for a component cleavage polyadenylation factor CF I, one four factors needed pre-mRNA 3'-end processing. show I copurify anion-exchange chromatography. These data suggest physically I. Extracts from thermosensitive pab1 mutant wild-type strain immunoneutralized showed normal activity large increase tail length. A length was restored by adding recombinant to extract. The longer were not due an inhibition exonuclease activities. has previously been implicated regulation translation initiation cytoplasmic mRNA stability. Our indicate also part RNA-processing complex thus participates control lengths during reaction.