Studies on the Polymer of Adenosine Diphosphate Ribose

摘要: A particulate fraction obtained from rat liver nuclei is capable of polymerizing the adenosine diphosphate ribose moiety nicotinamide adenine dinucleotide with simultaneous release nicotinamide. Comparative studies on this reaction and mononucleotide-dependent triphosphate polymerization, originally described by Chambon, Weill, Mandel, later confirmed partly Fujimura Sugimura, reveal that both reactions are fundamentally identical NAD but not ATP immediate substrate for polymerization. The activity, as assayed substrate, essentially localized in various organs markedly decreased prior incubation nuclear preparation pancreatic deoxyribonuclease ribonuclease T1 RNase, nor it affected addition actinomycin D or any nucleoside triphosphates. product resistant to either mild alkaline hydrolysis, DNase, Neurospora NADase, proteinases. Upon treatment venom phosphodiesterase, more than 95% material made acid-soluble produce a single compound possessing 1 mole adenine, 2 moles ribose, phosphate. Preliminary analysis suggests may be hitherto unknown macromolecule composed repeating ADP-ribose units.