Immortalization of plasma cells by plasmid DNA and its hybridoma
作者： Taizaburo Kanki , Sinichi Takeuchi
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摘要: Human plasma cell lines producing IgG were cloned by the limiting dilution method from polyclonally immortalized B-celllines with plasmid DNA (pSVTLbsr) containing simian virus 40 (SV40 )-gene primary peripheral blood mononuclear cells of healthy and nonimmune volunteers. The fused well conventional partner became evenly JgG-producing hybridomas at high efficiency, especially human origin. One difficulties in obtaining stable IgG-producing hybridoma using Epstein-Barr (EBV) transformation followed back fusion cells, might mainly be attributed to inability immortalize on account very low density CD21 (EBV receptor) surface. present CD2I-independent immortalization infection SV40 protein-coated will a potentially effective obtain monoclonal antibodies. [Hum Antibod Hybridomas 1995; 6: 89-92J
sci-hub.se 参考文章(13)
Keith James, Graham Thomas Bell, Human monoclonal antibody production: Current status and future prospects Journal of Immunological Methods. ,vol. 100, pp. 5- 40 ,(1987) , 10.1016/0022-1759(87)90170-0
C Chen, H Okayama, High-efficiency transformation of mammalian cells by plasmid DNA. Molecular and Cellular Biology. ,vol. 7, pp. 2745- 2752 ,(1987) , 10.1128/MCB.7.8.2745
F. C. Jensen, A. J. Girardi, R. V. Gilden, H. Koprowski, INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS Proceedings of the National Academy of Sciences of the United States of America. ,vol. 52, pp. 53- 59 ,(1964) , 10.1073/PNAS.52.1.53
Charles Cunningham, Monoclonal antibody and immunosensor technology: Ailsa M. Campbell, Elsevier, 1991. Dfl115.00 (pbk) (xxv + 427 pages) ISBN 0 444 81412 4 Trends in Biotechnology. ,vol. 10, pp. 330- ,(1992) , 10.1016/0167-7799(92)90259-X
F.L. Graham, A.J. Van Der Eb, Transformation of rat cells by DNA of human adenovirus 5 Virology. ,vol. 54, pp. 536- 539 ,(1973) , 10.1016/0042-6822(73)90163-3
D. Kozbor, W. Abramow-Newerly, P. Tripputi, S.P.C. Cole, J. Weibel, J.C. Roder, C.M. Croce, Specific immunoglobulin production and enhanced tumorigenicity following ascites growth of human hybridomas. Journal of Immunological Methods. ,vol. 81, pp. 31- 42 ,(1985) , 10.1016/0022-1759(85)90119-X
D. Bron, M. B. Feinberg, N. N. Teng, H. S. Kaplan, Production of human monoclonal IgG antibodies against Rhesus (D) antigen Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 3214- 3217 ,(1984) , 10.1073/PNAS.81.10.3214
Danuta Kozbor, John C. Roder, In vitro stimulated lymphocytes as a source of human hybridomas. European Journal of Immunology. ,vol. 14, pp. 23- 27 ,(1984) , 10.1002/EJI.1830140105
L E Strike, B H Devens, R L Lundak, Production of human-human hybridomas secreting antibody to sheep erythrocytes after in vitro immunization. Journal of Immunology. ,vol. 132, pp. 1798- 1803 ,(1984)
Y Ueki, I S Goldfarb, N Harindranath, M Gore, H Koprowski, A L Notkins, P Casali, Clonal analysis of a human antibody response. Quantitation of precursors of antibody-producing cells and generation and characterization of monoclonal IgM, IgG, and IgA to rabies virus. Journal of Experimental Medicine. ,vol. 171, pp. 19- 34 ,(1990) , 10.1084/JEM.171.1.19