Factors Contributing to Variability of Quantitative Viral PCR Results in Proficiency Testing Samples: a Multivariate Analysis

摘要: While viral load testing has gained widespread acceptance, a primary limitation remains the variability of results, particularly between different laboratories. some work demonstrated importance standardized quantitative control material in reducing this variability, little been done to explore other important factors molecular amplification process. Results 185 laboratories enrolled College American Pathologists (CAP) 2009 proficiency (PT) survey (VLS) were examined. This included 165 labs (89.2%) for cytomegalovirus (CMV), 99 (53.5%) Epstein-Barr virus (EBV), and 64 (34.6%) BK (BKV). At time PT, asked series questions characterize their methods. The responses these correlated mean (MVL) result (RV). Contribution individual RV was estimated through analysis variance (ANOVA) modeling use backward selection fit those models. Selection calibrator, commercially prepared primers probes, target gene found most prominently associated with changes MVL or one more viruses studied. Commercially probes made largest contribution overall variability. Factors contributing differed among viruses, as did relative each factor marked seen clinical results is multiple aspects design performance. reduction such will require multifaceted approach improve accuracy, reliability, utility tests.