The Epstein-Barr virus EBNA2 protein induces a subset of NOTCH target genes in thyroid cancer cell lines but fails to suppress proliferation
作者： Sean Golden , Xiao-Min Yu , Scott Odorico , Vansh Jain , Ana Marin
DOI: 10.1016/J.SURG.2016.06.068
关键词:
摘要: Background Epstein-Barr virus is associated with lymphoid and epithelial malignancies has been reported to infect thyroid cells. The protein, EBNA2, regulates viral cellular promoters by binding RBP-jκ. Similarly, NOTCH1, a tumor suppressor protein in cells, competes EBNA2 for overlapping sites on activates subset of NOTCH-responsive genes lymphocytes myocytes; however, the effect expression NOTCH targets cells unknown. Here we have explored whether NOTCH1 cancer lines examined its proliferation. Methods Two human lines, follicular FTC-236 anaplastic HTh7, were transfected or control vectors. Notch measured using quantitative reverse transcriptase polymerase chain reaction. Cellular proliferation was MTT analysis. Results activated only targets. Expression HES1 HEY1 increased 10-fold HTh7 respectively, but majority not affected. In contrast did suppress Conclusion does activate most Notch1-responsive Instead, may compete limiting amounts RBP-jκ inhibit certain aspects signaling.
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