Biochemical and immunological studies of purified mouse beta-galactosidase.
作者： S Tomino , M Meisler
DOI: 10.1016/S0021-9258(19)40879-X
关键词:
摘要: Beta-Galactosidase (EC 3.2.1.23) has been purified from the livers of C57BL/6J mice. The enzyme migrated as a single band protein on polyacrylamide gel electrophoresis in presence sodium dodecyl sulfate. molecular weight denatured and reduced was 63,000. native form beta-galactosidase appeared to be tetramer 240,000 at pH 5.0, which reversibly dissociated alkaline dimer with apparent 113,000. Multiple charge isomers were resolved by ion exchange chromatography. Treatment neuraminidase markedly its electrophoretic mobility. Purified well crude liver extract hydrolyzed p-nitrophenyl-beta-D-fucoside one-tenth rate hydrolysis beta-galactoside. Antiserum precipitated major portion activity mouse liver, brain, kidney. This antiserum cross-reacts beta-galactosidases rat Chinese hamster, but not human, porcine, or bovine beta-galactosidase.
sci-hub.st 参考文章(18)
M Meisler, M C Rattazzi, Immunological studies of beta galactosidase in normal human liver and in GM1 gangliosidosis. American Journal of Human Genetics. ,vol. 26, pp. 683- 691 ,(1974)
Reza Seyedyazdani, Lars-G. Lundin, Genetic relationship between beta-galactosidase and beta-fucosidase in the mouse. Biochemical Genetics. ,vol. 12, pp. 441- 448 ,(1974) , 10.1007/BF00486061
Richard R. Burgess, Separation and characterization of the subunits of ribonucleic acid polymerase. Journal of Biological Chemistry. ,vol. 244, pp. 6168- 6176 ,(1969) , 10.1016/S0021-9258(18)63521-5
Anthony G.W. Norden, Linda L. Tennant, John S. O'Brien, G1 Ganglioside β-Galactosidase A Journal of Biological Chemistry. ,vol. 249, pp. 7969- 7976 ,(1974) , 10.1016/S0021-9258(19)42060-7
C-C Wang, O Touster, Turnover studies on proteins of rat liver lysosomes. Journal of Biological Chemistry. ,vol. 250, pp. 4896- 4902 ,(1975) , 10.1016/S0021-9258(19)41253-2
James Felton, Miriam Meisler, Kenneth Paigen, A Locus Determining β-Galactosidase Activity in the Mouse Journal of Biological Chemistry. ,vol. 249, pp. 3267- 3272 ,(1974) , 10.1016/S0021-9258(19)42667-7
Jack J. Distler, George W. Jourdian, The Purification and Properties of β-Galactosidase from Bovine Testes Journal of Biological Chemistry. ,vol. 248, pp. 6772- 6780 ,(1973) , 10.1016/S0021-9258(19)43421-2
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
M. Meisler, K. Paigen, Coordinated Development of β-Glucuronidase and β-Galactosidase in Mouse Organs Science. ,vol. 177, pp. 894- 896 ,(1972) , 10.1126/SCIENCE.177.4052.894
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)