The protein tyrosine phosphatase DEP-1 is induced during differentiation and inhibits growth of breast cancer cells.

摘要: Sodium butyrate-induced differentiation of breast cancer cell lines was used to identify protein tyrosine phosphatases (PTPs) involved in and growth inhibition cells. Of 42 PTPs analyzed, 31 were expressed the ZR75-1 line. Expression four (DEP-1, SAP, PTP gamma, PAC) regulated cells undergoing differentiation. two these (DEP-1 SAP) also SKBr-3 line In view its marked induction with an estrogen receptor (ER)-positive ER-negative line, DEP-1 investigated for a role or A cDNA construct under control constitutively active cytomegalovirus promoter transfected into ZR75-1, SKBR-3, MCF-7 lines, resistant colonies selected G418. expression inhibited development by 3-5-fold all three compared transfection vector alone. Three stable expressing inducible metallothionein then established. 5-10-fold. These data describe during one PTP, DEP-1, that inhibits vitro.