Automated screening procedure for high-throughput generation of antibody fragments.

作者: Johan Hallborn , Roland Carlsson

DOI: 10.2144/DEC02-HALLBORN

关键词:

摘要: In the emerging field of proteomics, there is an urgent need for catcher molecules such as antibodies detecting proteome or parts in a microarray format. A suitable source providing large diversity binders obtained by combinatorial libraries, phage display libraries single chain antibody fragments (scFv) Fab fragments. To find novel from n-CoDeR with high throughput, we have automated screening process robotics. The system configured to screen tens thousands clones per day target antigens various formats, including peptides and soluble proteins, well cell-bound targets; thus, it designed meet demands proteomics area.

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