Membrane-Associated-Proteins: Self-Assembly, Interactions, and Biomolecular Materials

摘要: Membrane-associated-proteins are active components of biological membranes facilitating functions such as energy conversion, molecular transport, and recognition. Two-dimensional (2D) self-assemblies membrane-proteins bacteriorhodopsin bacterial proteins studied (1) from the interest in elucidation their structure-function correlations, (2) for development biomolecular materials. In these lectures we describe a first series recent high resolution synchrotron x-ray scattering studies purple membrane comprised 2D self-assembly membrane-protein (bR). At humidity, lattice undergoes reversible melting transition. The ordered phase this multi- component highly complex is found to exhibit characteristic solid with power-law decay positional correlations. Two significant consequences measurements protein-lattice elastic rigidity which directly derived protein-protein interactions, realization theoretical limit on achievable protein crystallography two- dimensional crystals correlations lack true long-range order. low humidity regime, remarkable exceedingly temperature bR has been discovered. Higher order stacked membranes, achieved by water removal, result complete suppression transition, absence denaturation up 140 °C.