CUG-BP binds to RNA substrates and recruits PARN deadenylase.

摘要: CUG-BP is the human homolog of Xenopus EDEN-BP, which was shown previously to bind mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization oocyte. While several studies have focused on roles a splicing or translation regulator in mammalian cells, its role mRNA decay has not been examined detail. Here, we used an vitro assay dissect function two ARE-containing mRNAs: c-fos and TNFa. binds specifically both these RNAs stimulates poly(A) shortening by PARN. Moreover, interacts with PARN extracts coimmunoprecipitation, this interaction can be recapitulated using recombinant proteins. CUG-BP, therefore, first RNA-binding protein directly recruit deadenylase RNA substrate.