Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces
作者： Robert F. Potter , Alaric W. D'Souza , Meghan A. Wallace , Angela Shupe , Sanket Patel
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摘要: Two Gram-negative bacilli strains, designated BP-1(T) and BP-2, were recovered from two different Intensive Care Unit surfaces during a longitudinal survey in Pakistan. Both strains unidentified using the bioMerieux VITEK MS IVD v2.3.3 Bruker BioTyper MALDI-TOF mass spectrometry platforms. To more precisely determine taxonomic identity of we employed biochemical phylogenomic approach. The 16S rRNA gene sequence strain had highest to Citrobacter farmeri CDC 2991-81(T) (98.63%) amalonaticus CECT 863(T) (98.56%), sedlakii NBRC 105722(T) (97.74%) rodentium 105723(T) (97.74%). utilization scheme Analytic Profile Index for Enterobacteriaceae (API20E) indicated its enzymatic functions are unique within but most closely resemble Kluyvera spp., Enterobacter cloacae koseri/farmeri. Phylogenomic analysis shared genes between BP-1(T), BP-2 type Kluyvera, Citrobacter, Escherichia, Salmonella, Kosakonia, Siccibacter Shigella indicate that isolates form distinct branch these genera. Average Nucleotide Identity indicates same species. represent novel species new genus family, which name Superficieibacter electus gen. nov., sp. is proposed. (= ATCC BAA-2937, = 113412).
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