Determinação simultânea de chumbo e cádmio em sangue por espectrometria de absorção atômica com forno de grafite

摘要: Blood lead and cadmium levels have been used to evaluate recent human exposure these metals. Graphite Furnace Atomic Absorption Spectrometry (GFAAS) is the most suitable technique for determinations in biological fluids due its ability detecting low concentrations of metals, using small amount sample. The objective this study was establish validate a methodology simultaneous determination blood by GFAAS technique. samples were diluted with Triton X-100 HNO 3 , mixture NH 4 H 2 PO Mg(NO ) solutions as chemical modifier. analytical conditions optimized best pyrolysis atomization temperatures 500oC 1500oC, respectively. For validation, certified reference materials NIST SRM 955b (Lead bovine blood) 966 (Toxic metals used. evaluated parameters were: linearity (5.0 100.0 µg.L -1 0.3 3.0 ), accuracy (99.9% 97.7%), precision (RSD = 3% 13%), detection limit (0.54 0.06 quantification (1.8 0.21µg.L proposed method fast, sensitive from populations occupational or environmental exposure, including children.