Specific in situ cleavage of 16S ribosomal RNA of Escherichia coli interferes with the function of initiation factor IF-1

摘要: Abstract Specific in situ cleavage of 16S rRNA E. coli has been accomplished by vitro treatment 70S ribosomes ("tight couples") with the bacteriocin cloacin DF13. The defective ribosomes, which have fully lost their ability to sustain polypeptide synthesis, are still able form initiation on complexes MS2 RNA, but kinetics altered. This is apparently due an improper functioning factor IF-1, for ribosomal couples respond normally dissociation IF-3 not stimulated IF-1. formed reactive puromycin. Their bind alanyl-tRNA reduced about 50% at all concentrations elongation Tu studied. Cleavage rRNA, release terminal fragment from ribosome, causes block protein synthesis and aberrations observed during elongation.