Functional and stable expression of recombinant human FOXP3 in bacterial cells and development of antigen-specific monoclonal antibodies.

作者: S. Hu , J. Dai , H. Wei , K. Fan , H. Wang

DOI: 10.1093/JB/MVM160

关键词:

摘要: … to an OD 600 of 1.2 before adding 0.5 mM IPTG, and culturing the cells for an additional 3 h. … of the expressed fusion protein, we next assayed the recombinant protein for DNA-binding …

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