Antigen-specific T cells with monogamous or promiscuous restriction patterns are sensitive to different HLA-DR beta chain substitutions.

摘要: The contributions of the amino acids at 13 polymorphic positions in HLA-DR7 beta 1 chain to T cell recognition two antigenic peptides tetanus toxin (p2 and p30) were assessed using transfectants expressing mutant DR7 chains as APC for six toxin-specific clones with different restriction patterns: monogamous (restricted by only) or promiscuous DR7; DR1; DR2, Dw21; DR4, Dw4). Each substitutions significantly decreased eliminated ability molecule present a peptide one more clones, but none abolished all clones. Interestingly, 4 25, which are predicted class II model be located outside binding groove, Ag some not others. four specific p2 p30 had reactivity pattern panel mutants, indicating that TCR each clone has view p2/DR7 p30/DR7 complex. These data emphasize complexity interactions multiple residues Ag-specific recognition.