Brenneria goodwinii sp. nov., associated with acute oak decline in the UK.

作者: Sandra Denman , Carrie Brady , Susan Kirk , Ilse Cleenwerck , Stephanus Venter

DOI: 10.1099/IJS.0.037879-0

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摘要: A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms acute decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form distinct lineage within genus Brenneria , family Enterobacteriaceae , are most closely related to Brenneria rubrifaciens (97.6 % sequence similarity type strain). Multilocus based on four housekeeping genes (gyrB, rpoB, infB atpD) confirmed their position while DNA–DNA hybridization indicated belong single taxon. The can be differentiated phenotypically closest neighbours. phenotypic data demonstrate with AOD represent novel species for which name Brenneria goodwinii sp. nov. (type strain FRB 141T  = R-43656T  = BCC 845T  = LMG 26270T  = NCPPB 4484T) is proposed.

参考文章(20)
S. Denman, J. Webber, Oak declines: new definitions and new episodes in Britain. Quarterly Journal of Forestry. ,vol. 103, pp. 285- 290 ,(2009)
Kate Wilson, Preparation of Genomic DNA from Bacteria Current protocols in molecular biology. ,vol. 56, ,(2001) , 10.1002/0471142727.MB0204S56
S. Niemann, A. Pühler, H.‐V. Tichy, R. Simon, W. Selbitschka, Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population Journal of Applied Microbiology. ,vol. 82, pp. 477- 484 ,(1997) , 10.1046/J.1365-2672.1997.00141.X
Dries Vansteenkiste, Luc Tirry, Joris Van Acker, Marc Stevens, Predispositions and symptoms of Agrilus borer attack in declining oak trees Annals of Forest Science. ,vol. 61, pp. 815- 823 ,(2004) , 10.1051/FOREST:2004076
Elena G. Biosca, Raquel González, María José López-López, Santiago Soria, Carmina Montón, Eduardo Pérez-Laorga, María M. López, Isolation and Characterization of Brenneria quercina, Causal Agent for Bark Canker and Drippy Nut of Quercus spp. in Spain Phytopathology. ,vol. 93, pp. 485- 492 ,(2003) , 10.1094/PHYTO.2003.93.4.485
Johan Goris, Ken-ichiro Suzuki, Paul De Vos, Takashi Nakase, Karel Kersters, Evaluation of a microplate DNA : DNA hybridization method compared with the initial renaturation method Canadian Journal of Microbiology. ,vol. 44, pp. 1148- 1153 ,(1998) , 10.1139/W98-118
Lysiane Hauben, Edward R.B. Moore, Luc Vauterin, Marijke Steenackers, Joris Mergaert, Linda Verdonck, Jean Swings, Phylogenetic Position of Phytopathogens within the Enterobacteriaceae Systematic and Applied Microbiology. ,vol. 21, pp. 384- 397 ,(1998) , 10.1016/S0723-2020(98)80048-9
C. Poza-Carrión, I. Aguilar, F. J. Gallego, Y. Nuñez-Moreno, E. G. Biosca, R. González, M. M. López, P. Rodríguez-Palenzuela, Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and development of PCR primers Plant Pathology. ,vol. 57, pp. 308- 319 ,(2008) , 10.1111/J.1365-3059.2007.01778.X
Carrie Brady, Sandra Denman, Susan Kirk, Stephanus Venter, Pablo Rodríguez-Palenzuela, Teresa Coutinho, Description of Gibbsiella quercinecans gen. nov., sp. nov., associated with Acute Oak Decline. Systematic and Applied Microbiology. ,vol. 33, pp. 444- 450 ,(2010) , 10.1016/J.SYAPM.2010.08.006