The use of low temperature X-ray diffraction to evaluate freezing methods used in freeze-fracture electron microscopy.

摘要: SUMMARY Two methods of freezing samples for freeze-fracture electron microscopy have been compared using X-ray diffraction and results from lipid-water model systems. Perturbations the molecular organization hydrocarbon chains extent ice crystal formation evaluated lamellar phases egg lecithin containing 16% water lecithin-phosphatidylinositol 55% water, both freeze-quenched in liquid Freon-22 near its melting temperature (113 K). Very thin sandwiched between copper sheets separated by an microscope grid show much less induced structural rearrangement than small contained on conventional Balzers-type gold planchettes. These that rate very preparations is greater ones, which probably due part to improved dissipation heat a poorly conductive sample through highly sheets.